Rabbit anti-CTCF Recombinant Monoclonal Antibody

SKU: orb1519894

Description

CTCF Antibody

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, SW-Size, WB
• Dilution range: WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR041F
• Immunogen: Between 677 and C-terminus
• Target: CTCF
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Disclaimer: For research use only

Alternative Names

CTCFL paralog; MRD21; CCCTC-binding factor (zinc finger protein); CCCTC-binding factor; 11-zinc finger protein; 11 zinc finger transcriptional repressor; transcriptional repressor CTCF

Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat (10 µg) , Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519894) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519894) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519894). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519894). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519894) used for IP at 20 µl/mg lysate.

Detection of mouse CTCF by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519894). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519894). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CTCF

UniProt

P49711

RefSeq (Protein):NP_006556.1