Cart summary

You have no items in your shopping cart.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Catalog Number: orb1519973

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb1519973
CategoryAntibodies
DescriptionCD45 Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBL-178-12C7
Tested applicationsFC, ICC, IHC, IP, WB
ReactivityHuman
ImmunogenBetween 1256 and C-terminus
Antibody TypePrimary Antibody
Concentration50 µg/ml
Dilution rangeWB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetCD45
UniProt IDP08575
NCBINP_002829.3
Storage2 - 8°C
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Alternative namesL-CA; protein tyrosine phosphatase, receptor type,
Read more...
NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973) used for IP at 20 µl/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (orb1519973). For blotting immunoprecipitated CD45, orb1519973 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Rabbit anti-CD45 Recombinant Monoclonal Antibody

Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

  • Rabbit anti-CD45 Recombinant Monoclonal Antibody [orb1519975]

    FC,  ICC,  IHC,  IP,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μl
  • Recombinant CD45RB (B-Cell Marker) Antibody [orb389238]

    FC,  IF,  IHC,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    100 μg
  • CD45 Recombinant Monoclonal Antibody [orb418643]

    ELISA

    Human

    Monoclonal

    Unconjugated

    50 μl, 100 μl
  • Rabbit anti-CD45 Recombinant Monoclonal Antibody [orb1519974]

    FC,  ICC,  IHC,  IP,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    100 μg