Rabbit anti-CD3E Recombinant Monoclonal Antibody

• Catalog Number: orb1519962
• Category: Antibodies
• Description: CD3E Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BL-298-5D12
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 157 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD3E
• UniProt ID: P07766
• NCBI: NP_000724.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CD3-epsilon; TCRE; T-cell surface glycoprotein CD3
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD3E (orange) , CD8 alpha (red) , and PD-L1 (green) in FFPE breast carcinoma by IHC-IF. Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (orb1519962) , rabbit anti-CD8 alpha recombinant monoclonal, rabbit anti-PD-L1 recombinant monoclonal. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).