Rabbit anti-Cbl-b Antibody

SKU: orb1525792

Description

Cbl-b Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 475 and 525
• Target: Cbl-b
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

RING-type E3 ubiquitin transferase CBL-B; signal transduction protein CBL-B; RNF56; RING finger protein 56; Nbla00127; Cbl-b; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cbl proto-oncogene B, E3 ubiquitin protein ligase; casitas B-lineage lymphoma proto-oncogene b; Cas-Br-M (murine) ecotropic retroviral transforming sequence b; SH3-binding protein CBL-B; E3 ubiquitin-protein ligase CBL-B

Detection of human Cbl-b by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody orb1525792 used for IP at 3 µg/mg lysate.

Detection of human Cbl-b by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cbl-b (Cat. No. orb1525792) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

Cbl-b

UniProt

Q13191

RefSeq (Protein):NP_733762.2