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PRDM14 Antibody

SKU: orb1256971

Description

PRDM14 Antibody

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein of mouse PRDM14
TargetPRDM14
Molecular WeightObserved: 64kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PR domain zinc finger protein 14, PR domain-containing protein 14

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PRDM14 Antibody

Western blot analysis of extracts of Mouse liver, using PRDM14 antibody (orb1256971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

PRDM14 Antibody

Immunofluorescence analysis of HeLa cells using PRDM14 antibody (orb1256971) at dilution of 1:100. Blue: DAPI for nuclear staining.

PRDM14 Antibody

Immunofluorescence analysis of NIH/3T3 cells using PRDM14 antibody (orb1256971) at dilution of 1:100. Blue: DAPI for nuclear staining.

PRDM14 Antibody

Immunofluorescence analysis of PC12 cells using PRDM14 antibody (orb1256971) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

PRDM14 Antibody (orb1256971)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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