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Product Overview
Product Name PAI-1 antibody
Catalog Number orb303829
Tested applicationsELISA, IHC-P, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1
Target PAI-1
Product Properties
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Purity Purified by affinity chromatography
Uniprot ID P05121 (Human)
Product Description

Rabbit polyclonal antibody to PAI-1

Application Notes
Dilution Range WB: 0.1-0.2 μg/ml, IHC-P: 62.5 ng/ml
Application Notes WB Notes: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

*Additional Immunostaining data available. Please contact Tech Support for information.
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