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Catalog Number: orb303829
|Product Name||PAI-1 antibody|
|Tested applications||ELISA, IHC-P, WB|
|Immunogen||Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPAI-1|
|Form/Appearance||A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Storage||Store at 4°C for short term and at-20°C for long term storage.|
|Note||For research use only.|
|Purity||Purified by affinity chromatography|
|Uniprot ID||P05121 (Human)|
Rabbit polyclonal antibody to PAI-1
|Dilution Range||WB: 0.1-0.2 μg/ml, IHC-P: 62.5 ng/ml|
|Application Notes||WB Notes: To detect hPAI-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPAI-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
ELISA Notes: To detect hPAI-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
*Additional Immunostaining data available. Please contact Tech Support for information.
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