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KLH antibody (Biotin)

Catalog Number: orb345884

DispatchUsually dispatched within 5-10 working days
$ 737.00
Catalog Numberorb345884
CategoryAntibodies
DescriptionKLH antibody (Biotin)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityMollusc
IsotypeIgG
ImmunogenKLH (Keyhole Limpet Hemocyanin
Concentration1.0 mg/mL
Dilution rangeELISA: 1:250,000, IHC: User Optimized, WB: 1:5,000 - 1:25,000
Form/AppearanceLyophilized
PurityAnti-KLH Antibody biotin conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin, and KLH.
ConjugationBiotin
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit Anti-KLH antibody biotin conjugation, bioti
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NoteFor research use only
Application notesAnti-KLH Biotin conjugated Antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Anti-KLH antibody biotin conjugate is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
Expiration Date12 months from date of receipt.

Western blot analysis of KLH reduced (Lane1), none (Lane2) Load: 1 ug per lane using KLH antibody (Biotin)

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