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Catalog Number | orb764238 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Neu (phospho-Tyr1221/Y1222) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240 |
MW | 180 |
Tested applications | ELISA, IHC-P, IP, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-ERBB2 antibody, anti-HER2 antibody, anti-MLN1 Read more... |
Note | For research use only |
Western Blot analysis of various cells using Phospho-Neu (Y1221/Y1222) Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr1222) Antibody. The lane on the right is blocked with the phospho peptide.
ELISA, IF, IHC-P, WB | |
Human, Monkey, Mouse, Rat | |
Polyclonal | |
Unconjugated |