Myc Tag Mouse Monoclonal Antibody

SKU: orb544570

Description

Myc Tag Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:5000-20000, ELISA=1:1000-5000
• Reactivity: All

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 9E10
• Immunogen: KLH conjugated synthetic peptide derived from human Myc Tag
• Target: MYC
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc; proto-oncogene c-Myc; protooncogene homologous to myelocytomatosis virus; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog(avian).

Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45-50 kDa.

Sample: Lane 1: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Lane 2: Myc-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Primary: Anti-Myc tag (orb544570) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.

Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody (Cat. orb544570). orb544570 was diluted at 1:2000. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20 ug.

Quick Database Links

Gene Symbol

MYC