MUC5AC Antibody

SKU: orb2638933

Description

Mucin 5AC glycoprotein (MUC5AC) is a HMW glycoprotein belonging to the superfamily of mucins. Mucins are produced by epithelial cells and can be divided into two families; secretory mucins and membrane bound mucins. MUC5AC is a mucus-forming, secreted mucin that is found in normal gastric and tracheo-bronchial mucosa, but absent from normal colon. MUC5AC expression is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the MUC5AC antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CLH2
• Immunogen: A synthetic peptide of human MUC5AC tandem repeat was used as the immunogen for the MUC5AC antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

IHC testing of FFPE human gastric carcinoma with MUC5AC antibody (clone CLH2). Required HIER: steam section in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool prior to testing.

SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone CLH2). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P98088