TNF alpha antibody

• Catalog Number: orb371962
• Category: Antibodies
• Description: TNF alpha antibody
• Target: TNF alpha
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human TNF alpha. Please contact us for the exact immunogen sequence. The peptide is available as orb374984.
• UniProt ID: P01375, P06804, P16599
• MW: 27 kDa
• Tested applications: ICC, IF, IHC-P
• Dilution range: IF/ICC: 1:100-800, WB: 1:100-2000, IHC-P: 1:100-800 (based on 0.5 mg/ml)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-APC1 antibody, anti-Cachectin antibody, anti-
• Note: For research use only
• Entrez: 7124
• NCBI: 000585, 000594
• Expiration Date: 12 months from date of receipt.

References

H Keyhan, H Morovvati, MK Koohi, J Hassan, M Mazidi Hematological and Histological Changes in the Knee Cartilage of Male Rats Received Intra-Articular Injection of Bee Venom and Melittin journals.ut.ac.ir, (2025)

PubMed:

Mehmet Berker 1, Sibel Köktürk 1, Sibel Doğan 1, Emel Usta Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study Ultrastruct Pathol, (2025)

PubMed: 40247639

Applications

IHC

Reactivity

Human

Mahshid Kharaziha 1 2, Sahar Salehi 2, Mahshid Shokri 1, Seyed Mohsen Ahmadi Tafti 3 4, Thomas Scheibel 2 5 6 7 8 ROS-Scavenging Multifunctional Microneedle Patch Facilitating Wound Healing Adv Healthc Mater, (2025)

PubMed: 40832795

Applications

IHC

Reactivity

Human

IHC-P image iof human breast tumour using TNF alpha antibody (2.5 ug/ml)

Immunofluorescense analysis of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

IF analysis of mouse uterus tissue using anti-TNF alpha (2.5 ug/ml)

Immunohistochemical staining of mouse uterus tissue using TNF alpha antibody (2.5 ug/ml)

Immunofluorescense analysis of rat skin tissue using TNF alpha antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat liver tissue using anti-TNF alpha (2.5 ug/ml)