TNF alpha antibody

• Catalog Number: orb11495
• Category: Antibodies
• Description: TNF alpha antibody
• Target: TNF alpha
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Purified by Protein A affinity chromatography.
• Immunogen: KLH conjugated synthetic peptide derived from human TNF alpha. Please contact us for the exact immunogen sequence. The peptide is available as orb374753.
• UniProt ID: P01375, P06804, P16599
• RRID: AB_10748636
• MW: 27 kDa
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: IHC-P: 1:100, WB: 1:100-800
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti APC1 antibody, anti Cachectin antibody, anti
• Note: For research use only
• Entrez: 7124
• NCBI: 000594, 21, 000585, 31
• Expiration Date: 12 months from date of receipt.

References

Park, Byung-Joo et al. Analysis of the Immune Responses in the Ileum of Gnotobiotic Pigs Infected with the Recombinant GII.p12_GII.3 Human Norovirus by mRNA Sequencing Viruses, 13, 92 (2021)

PubMed: 33440894

Applications

WB

Reactivity

Porcine

Eduardo Quintão Manhanini Souza # 1, Luan Felipe Toro # 2 3, Vinícius Franzão Ganzaroli 1, Jéssica de Oliveira Alvarenga Freire 2 3, Mariza Akemi Matsumoto 2, Cláudio Aparecido Casatti 2, Luciano Tavares Ângelo Cintra 4, Rogério Leone Buchaim 5, João Paulo Mardegan Issa 6, Valdir Gouveia Garcia 7, Leticia Helena Theodoro 1, Edilson Ervolino Peri-implantitis increases the risk of medication-related osteonecrosis of the jaws associated with osseointegrated implants in rats treated with zoledronate Sci Rep., 14, 627

PubMed: 38182598

Applications

IHC

Reactivity

Rat

Romulo de Oliveira Sales-Junior 1, Bharbara de Moura Pereira 1, Rafaela Ricci 1, Nathália Evelyn da Silva Machado 1, Julissa Denisse Arguello Alvarado 1, Ana Beatriz Carreto 1, Edilson Ervolino 2, Anil Kishen 3, Luciano Tavares Ângelo Cintra 1, João Eduardo Gomes-Filho Systemic administration of polyphenols from dealcoholized red wine reduces inflammation and bone resorption in established apical periodontitis in male rats Arch Oral Biol ., (2025)

PubMed: 39986211

Applications

IHC

Reactivity

Rat

Juliano Milanezi de Almeida 1 2, Halef Diego Turini 1, Henrique Rinaldi Matheus 1 2 3, Otávio Augusto Pacheco Vitória 1 2, Bianca Rafaeli Piovezan 1 2, Ruan Henrique Barra Dalmonica 1 2, Elisa Mara de Abreu Furquim 1 2, Edilson Ervolino Omega-3 attenuates the severity of medication-related osteonecrosis of the jaws in rats treated with zoledronate PLoS One ., (2025)

PubMed: 40138277

Zolotarenko, Alena et al. FRA1 mediates the activation of keratinocytes: Implications for the development of psoriatic plaques Biochim Biophys Acta Mol Basis Dis, 1864, 3726-3734 (2018)

PubMed: 30318470

Applications

IF, WB

Reactivity

Human

Western blot analysis of rat liver tissue using TNF alpha antibody (primary antibody dilution at 2 ug/ml)

IF image of pig liver vessel tissue using TNF-alpha antibody (primary antibody at 1:100)

Immunohistochemical staining of paraffin embedded rat liver tissue using anti-TNF alpha (2.5 ug/ml)

Immunohistochemical staining of pig liver tissue using anti-TNF-alpha (dilution of primary antibody - 1:100)

Immunofluorescence image of mouse uterus tissue using TNF alpha antibody (2.5 ug/ml)

Western blot analysis of rat testis (lane 1), human thyroid tumour (lane 2) using TNF alpha antibody (1 ug/ml)

IHC-P staining of pig liver tissue using TNF alpha antibody (dilution at 1:100)