Custom Nanobody Development

 

Nanobodies, or single-domain antibodies (VHH) derived from camelid species (Alpaca/Llama), have gained significant attention in recent years due to their small size (15 kDa), high affinity, and robust stability. Our experienced team at Biorbyt is dedicated to delivering high-quality nanobodies customized to your unique requirements.

Service Highlights

Efficient Single B Platform

Utilizing our proprietary single B platform, we efficiently isolate and culture B cells from immunized Camelids, which enables rapid generation of high-quality nanobodies tailored to your specific targets.

Rapid VHH Library Generation

Within just 4 weeks, we can generate a comprehensive VHH library derived from the immunized Camelid B cells. This serves as a valuable resource for identifying candidates with exceptional binding affinity.

Accelerated Screening Process

Our optimized screening process allows for the identification of VHH hits within 2 months, significantly reducing the timeline compared to traditional hybridoma methods.

Optimization with Mammalian Display

Our nanobodies can be further optimized using our mammalian cell display-based antibody engineering system to enhance specific properties such as stability or affinity.

Development Phases

Our streamlined workflow ensures rapid delivery from immunization to validated VHH hits:

Stage	Service Details	Est. Turnaround
1. Camelid Immunization	Immunization of Alpacas or Llamas to elicit a strong immune response against the target antigen.	Standard (6-8 Weeks)
2. Single B Isolation	Isolation of specific B cells using our proprietary Single B platform.	1-2 Weeks
3. VHH Library Generation	Construction of a comprehensive VHH library derived from the immunized B cells.	4 Weeks
4. Screening & Identification	High-throughput screening to identify VHH hits with high specificity.	Within 2 Months
5. Optimization (Optional)	Engineering via mammalian cell display for enhanced affinity/stability.	Upon Request

Contact Us for Quotation