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Catalog Number | orb1258115 |
---|---|
Category | Antibodies |
Description | IRF2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 42kDa |
Target | IRF2 |
UniProt ID | P14316 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | IRF2, IRF-2 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using IRF2 antibody (orb1258115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using IRF2 antibody (orb1258115) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using IRF2 antibody (orb1258115) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IRF2 antibody (orb1258115) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IRF2 antibody (orb1258115) at dilution of 1:200 (40x lens).
IF, IHC-Fr, IHC-P | |
Bovine, Equine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ICC, WB | |
Mouse | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |