IRF2 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb526663
• Category: Antibodies
• Description: IRF2 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 5F2
• Tested applications: ICC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant human IRF2 protein, around C-terminal 100aa
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: IRF2
• UniProt ID: P14316
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DKFZp686F0244; Interferon regulatory factor 2; IRF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (IRF2) monoclonal Antibody, Unconjugated (orb526663) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (IRF2) monoclonal Antibody, Unconjugated (orb526663) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

PANC-1 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (IRF2) monoclonal Antibody, Unconjugated (orb526663) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Human lung tissue lysates, Primary: Anti-IRF2 (orb526663) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 39 kD, Observed band size: 50 kD.