Hsp70 antibody

SKU: orb556728

Description

Rabbit polyclonal antibody to HSP70

Images & Validation

• Tested Applications: ICC, IHC-P, IP, WB
• Dilution range: WB: 1:5000-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:500-1:1000
• Reactivity: Human, Monkey, Mouse, Other, Rat
• Application Notes:
  Positive control: Jurkat , Raji , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , Mouse brain , PC-12 , Rat brain , 293T

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP70 1A. The exact sequence is proprietary.
• Target: HSP70
• Molecular Weight: 70
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol
• Concentration: 0.73 mg/ml
• Disclaimer: For research use only

Alternative Names

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Immunofluorescence analysis of HeLa cells using HSP70 antibody

Immunohistochemical staining of H1299 xenograft using HSP70 antibody

Immunohistochemical staining of Rat brain using HSP70 antibody

Immunoprecipitation analysis of HeLa Whole cell lysate using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Western blot analysis of Jurkat(Lane 1), Raji(Lane 2), 293T(Lane 3), A431(Lane 4), HeLa(Lane 5), HepG2(Lane 6), H1299(Lane 7), HCT116(Lane 8), MCF-7(Lane 9), NT2D1(Lane 10), PC-3(Lane 11), U87-MG(Lane 12) using HSP70 antibody

Quick Database Links

Gene Symbol

HSP70

UniProt

P0DMV9, P0DMV8

References

Shahabi, Shirin et al. Evaluating the Efficacy of Irisin Injection in Mimicking the Molecular Responses Induced by Endurance Exercise in Mouse Liver Tissue journals.lww.com, (2024)

PubMed:

Applications

IHC

Reactivity

Mouse