F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin conjugated

• Catalog Number: orb348299
• Category: Antibodies
• Description: F(ab')2 Rabbit IgG (H&L) antibody (RPE)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, IF
• Reactivity: Rabbit
• Isotype: IgG F(ab')2
• Immunogen: Rabbit IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 0.5 mg/mL
• Dilution range: FC: 1:100 - 1:250, IF: 1:100 - 1:250
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Conjugation: RPE
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjuga
• Note: For research use only
• Application notes: F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Secondary Antibody has been tested by dot blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Fab2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated. Antigen: Row 1: Rabbit IgG Row 2: Goat IgG Row 3: Mouse IgG Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.4 ng Lane 5 - 2.5 ng Primary antibody: N/A Secondary antibody: Fab2 Goat anti-Rabbit IgG Antibody Phycoerythrin Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 1 hour at RT