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Catalog Number | orb348104 |
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Category | Antibodies |
Description | F(ab')2 Dog IgG (H&L) antibody (RPE) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF |
Reactivity | Canine |
Isotype | IgG F(ab')2 |
Immunogen | Dog IgG whole molecule |
Antibody Type | Secondary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | FC: 1:100 - 1:250, IF: 1:100 - 1:250 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c). |
Conjugation | RPE |
Storage | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit F(ab')2 Anti-Dog Phycoerythrin Conjugated A Read more... |
Note | For research use only |
Application notes | Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. |
Expiration Date | 12 months from date of receipt. |
Multiplex antigen detection of SARS-CoV-2-specific antibodies in sera from SARS-CoV-2-neutralizing antibody positive (NA-pos) but not NA-neg dogs and cats.