F(ab')2 Guinea Pig IgG (H&L) Antibody Phycoerythrin conjugated

• Catalog Number: orb348142
• Category: Antibodies
• Description: F(ab')2 Guinea Pig IgG (H&L) antibody (RPE)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, IF
• Reactivity: Guinea pig
• Isotype: IgG F(ab')2
• Immunogen: Guinea Pig IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 0.5 mg/mL
• Dilution range: IF: 1:100-1:250
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Conjugation: RPE
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Rabbit F(ab')2 Anti-Guinea Pig IgG Antibody Phycoe
• Note: For research use only
• Application notes: F(ab')2 Anti-Guinea Pig IgG Phycoerythrin Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of F(ab')2 Rabbit Anti-Guinea Pig IgG Antibody Phycoerythrin Conjugated. Guinea Pig IgG Whole Molecule (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23 ng. Antibody: F(ab')2 Rabbit Anti-Guinea Pig IgG Phycoerythrin Conjugate at 1.0 µg/ml at RT for 1hr. Block: Fluorescent Buffer (p/n orb348644) at RT for 30 mins.