CD4 Rabbit Polyclonal Antibody

• Catalog Number: orb182470
• Category: Antibodies
• Description: CD4 Rabbit Polyclonal Antibody
• Target: CD4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine
• Predicted Reactivity: Bovine, Canine, Guinea pig, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
• UniProt ID: P01730
• MW: 48 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 mole
• Research Area: CD Markers, Cell Biology, HIV, Immunology, Infecti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb182470), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (orb182470) at 1:4000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody at 1:300 for one hour at room temperature.

Sample: Lane 1: U937 (Human) Cell Lysate at 30 ug, Lane 2: HL-60 (Human) Cell Lysate at 30 ug, Lane 3: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD4 (orb182470) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 55 kD.