CD4 Rabbit Polyclonal Antibody (APC)

• Catalog Number: orb994212
• Category: Antibodies
• Description: CD4 Rabbit Polyclonal Antibody (APC)
• Target: CD4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: APC
• Reactivity: Human, Mouse, Porcine
• Predicted Reactivity: Bovine, Canine, Guinea pig, Rat, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CD4 (385-457/457aa)
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 mole
• Research Area: CD Markers, Cell Biology, HIV, Immunology, Infecti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb994212), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CD4 antibody (orb994212), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.