CD4 antibody

SKU: orb4830

Description

CD4 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Dilution range: IHC-P: 1:500, IF/ICC: 1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD4. Please contact us for the exact immunogen sequence. The peptide is available as orb374775.
• Target: CD4
• Molecular Weight: 51 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti-CD4 antibody, Anti-CD4 antigen (p55) antibody, Anti-CD4 antigen antibody, Anti-CD4 molecule antibody, Anti-CD4 receptor antibody, Anti-CD4+ Lymphocyte deficiency, included antibody, Anti-CD4_HUMAN antibody, Anti-CD4mut antibody, Anti-L3T4 antibody, Anti-Leu3 antibody, Anti-Ly-4 antibody, Anti-Lymphocyte antigen CD4 antibody, Anti-MGC165891 antibody, Anti-OTTHUMP00000238897 antibody, Anti-p55 antibody, Anti-T cell antigen T4 antibody, Anti-T cell antigen T4/LEU3 antibody, Anti-T cell differentiation antigen L3T4 antibody, Anti-T cell OKT4 deficiency, included antibody, Anti-T cell surface antigen T4/Leu 3 antibody, Anti-T cell surface antigen T4/Leu3 antibody, Anti-T cell surface glycoprotein CD4 antibody, Anti-T-cell surface antigen T4/Leu-3 antibody, Anti-T-cell surface glycoprotein CD4 antibody, Anti-W3/25 antibody, Anti-W3/25 antigen antibody

WB analysis of human breast cancer (lane 1), human thyroid cancer (lane 2), human endometrial cancer (lane 3), human ovarian cancer (lane 4) using CD4 antibody (1 ug/ml)

WB analysis of human breast tumor (lane 1), human mammary fibroma (lane 2), human breast cancer (lane 3) using CD4 antibody (1 ug/ml)

IHC-P image of human thyroid carcinoma tissue using CD4 antibody (2.5 ug/ml)

Western blot analysis of rat thymus (lane 1), rat liver (lane 2), mouse spleen (lane 3), mouse samll intestine (lane 4) using CD4 antibody (1 ug/ml)

Immunofluorescence image of mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD4 antibody (2.5 ug/ml)

IHC-P image of rat skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of rat skin tissue using anti-CD4 (2.5 ug/ml)

IHC-P staining of rat colon tissue using CD4 antibody (5 ug/ml)

IHC-P staining of human thyroid carcinoma tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of pig large intestines tissue using anti-CD4 (dilution of primary antibody - 1:200)

Immunohistochemical staining of paraffin embedded pig large intestines tissue using CD4 antibody (2.5 ug/ml)

IHC-P image of pig large intestines tissue using anti-CD4 (2.5 ug/ml)

Immunofluorescence analysis of mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded mouse skin tissue using CD4 antibody (2.5 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using anti-CD4 (5 ug/ml)

Western blot analysis of human breast tumor (lane 1), human mammary fibroma (lane 2), human breast cancer (lane 3) using CD4 antibody (1 ug/ml)

Quick Database Links

Gene Symbol

CD4

UniProt

P05540, P01730, P06332

NCBI Gene ID

920

RefSeq:000607, 11, 41, 000616

References

B. Sayyaf Dezfuli a, F. Pironi a, G. Castaldelli b, L. Giari b, M. Lanzoni b, K. Buchmann c, P.W. Kania c, G. Bosi Anguilla anguilla vs Contracaecum rudolphii: Granuloma allows host tolerance and parasite survival Aquaculture, (2024)

PubMed:

Applications

IHC

Mohammad Jahan-Mahin 1, Roya Askari 1, Amir Hossein Haghighi 1, Omid Khaiyat The effect of three types of water-based training protocols on thymus atrophy and specific indicators of cellular immune senescence in aged male rats Biogerontology, (2025)

PubMed: 39832052

Applications

IHC

Reactivity

Rat

Urszula Daniluk # 1, Agnieszka Świdnicka-Siergiejko # 2, Jarosław Daniluk 2, Małgorzata Rusak 3, Milena Dąbrowska 3, Katarzyna Guzińska-Ustymowicz 4, Anna Pryczynicz 4, Andrzej Dąbrowski 2 The development of pancreatic cancer is accompanied by significant changes in the immune response in genetically predisposed mice Front Oncol ., (2025)

PubMed: 40641916

Applications

IHC

Reactivity

Mouse

Romero-Moreno, Ricardo et al. The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis Nat Commun, 10, 4404 (2019)

PubMed: 31562303

Applications

IHC

Reactivity

Mouse

Su, Jiyan et al. Protective Effect of Pogostone on 2,4,6-Trinitrobenzenesulfonic Acid-Induced Experimental Colitis via Inhibition of T Helper Cell Front Pharmacol, 8, 829 (2017)

PubMed: 29204117

Applications

IHC

Reactivity

Rat