You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb763374 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to His-Tag |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Isotype | IgG1 |
Immunogen | Synthetic Peptide |
Concentration | 1mg/ml |
Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
Conjugation | Unconjugated |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1:3000 1: untransfected 293 cell lysate 2: transfected 293 cell lysate with His-tag fusion protein 3: IP (untransfected 293 + anti-His mAb + Protein G agarose) 4: IP (transfected 293 + normal Mouse IgG + Prote).
IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His).
2ug His fusion protein + Primary antibody dilution at 1: 1:5, 000 2: 1:10, 000.
1: Multi-Tag Recombinant protein 2: 293T transfected with His recombinant protein 3: Insect cell trandfected with His recombinant protein.
Unconjugated | |
90% | |
20.6 kDa | |
Cynomolgus IL-10 Protein, His Tag (orb1496244) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P79338). |
Filter by Rating