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HIF1A Antibody
SKU: orb1258844
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Signal Transduction
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human HIF1A. |
| Target | HIF1A |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
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Western blot analysis of extracts of mouse kidney, using HIF1A antibody (orb1258844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver cancer using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HIF1A
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HIF1A Antibody (orb1258844)
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