HIF1A Antibody

• Catalog Number: orb1258844
• Category: Antibodies
• Description: HIF1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human HIF1A.
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIF1A
• UniProt ID: Q16665
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse kidney, using HIF1A antibody (orb1258844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human liver cancer using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using HIF1A antibody (orb1258844) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using HIF1A antibody (orb1258844) at dilution of 1:100. Blue: DAPI for nuclear staining.