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Product Overview
Product Name FluorBright DNA Dye
Catalog Number orb90570
Tested applicationsPCR, WB
Hazard InformationNon-Toxic
Target DNA Dye
Alternative Names
Product Properties
Storage The product is stable for at least 6 months when stored at 2-8°C. Avoid exposure to temperatures greater than 37°C and protect from light.
Note For research use only.
Kit Components

This product has the following advantages:

1. Safe - Absence of mutagenicity and low toxicity (LC>5000mg/kg) as compared to Ethium Bromide.

2. Low Environmental Impact - Compliance with the Clean Water Act standards. No water pollution concern.

3. Sensitivity - High degree of sensitivity as Ethium Bromide.

4. Convenience - Ready to Use; Same application procedures as the 6X Loading Dye.

5. Speed - No de-staining requirement, low background value, and image displayed after coupling with the nucleic acid.

6. Compatibility - Use the Blue Light or UV to detect the signal; Broad compatibility range.

7. Economic - Non-hazardous product; No expenses required for the waste management.

Product Description

FluorBright DNA Dye is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. FluorGold Protein Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. FluorGold Protein Dye is the most sensitive stain available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment requiring a safe , non-hazardous alternative to Ethidium Bromide. Tracking Dyes: Bromophenol Blue, Xylene Cyanol FF, and Orange G.

Application Notes
Application Notes

1. Vortex FluorGold Protein Dye for 10 seconds prior to use.

2. Dilute 1 part FluorGold Protein Dye with 5 parts DNA sample and mix. Note: FluorGold Protein Dye must be added to DNA markers in order to visualize the ladder bands simultaneously with the sample after electrophoresis.

3. Load sample and run according to standard procedures.

4. After the electrophoresis, remove gel and place on UV or a visible-light transilluminator to immediately visualize bands.

5. Gels can be post-stained with Ethidium Bromide if desired.

Validation Images
Slower migrating species are indicative of a linear or relaxed circular vector resulting from DNA nicking or strand breaks.
Slower migrating species are indicative of a linear or relaxed circular vector resulting from DNA nicking or strand breaks.
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