E2F6 Antibody

• Catalog Number: orb1255346
• Category: Antibodies
• Description: E2F6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:100CHIP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 37kDa
• Target: E2F6
• UniProt ID: O75461
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: E2F6, E2F-6
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:100CHIP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using E2F6 antibody (orb1255346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse lung using E2F6 antibody (orb1255346) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using E2F6 antibody (orb1255346). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 3 ug E2F6 antibody (orb1255346). Western blot was performed from the immunoprecipitate using E2F6 antibody (orb1255346) at a dilution of 1:500.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using E2F6 antibody (orb1255346) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.