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E2F6 Antibody
SKU: orb1255346
Featured
Description
Research Area
Epigenetics & Chromatin, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human E2F6 (NP_937987.2). |
| Target | E2F6 |
| Molecular Weight | Observed: 37kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−E2F6, E2F-6
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Western blot analysis of extracts of various cell lines, using E2F6 antibody (orb1255346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using E2F6 antibody (orb1255346) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using E2F6 antibody (orb1255346). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of MCF-7 cells using 3 ug E2F6 antibody (orb1255346). Western blot was performed from the immunoprecipitate using E2F6 antibody (orb1255346) at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using E2F6 antibody (orb1255346) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
E2F6
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
E2F6 Antibody (orb1255346)
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