DDX58 Antibody

SKU: orb1274366

Description

DDX58 Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 830 to the C-terminus of human DDX58 (NP_055129.2).
• Target: DDX58
• Molecular Weight: Observed: 102kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

RIG-1 Antibody: RIGI, RIG-I, RLR-1, Probable ATP-dependent RNA helicase DDX58, DEAD box protein 58

Western blot analysis of extracts from normal (control) and DDX58 knockout (KO) A-549 cells, using DDX58 antibody (orb1274366) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Quick Database Links

Gene Symbol

DDX58

UniProt

O95786