DDX58 Antibody, KO Validated

• Catalog Number: orb1274366
• Category: Antibodies
• Description: DDX58 Antibody, KO Validated
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 830 to the C-terminus of human DDX58 (NP_055129.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 102kDa
• Target: DDX58
• UniProt ID: O95786
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: RIG-1 Antibody: RIGI, RIG-I, RLR-1, Probable ATP-d
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts from normal (control) and DDX58 knockout (KO) A-549 cells, using DDX58 antibody (orb1274366) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.