DBH/Dopamine Beta Hydroxylase Antibody

• Catalog Number: orb1539385
• Category: Antibodies
• Description: DBH/Dopamine Beta Hydroxylase Antibody
• Target: DBH/Dopamine Beta Hydroxylase
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Guinea pig, Hamster, Human, Mouse, Rabbit, Rat
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-DNHYSPHFQEIR, from the internal region of the protein sequence according to NP_000778.3.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line Jurkat (calculated MW of 69.1kD according to NP_000778.3). Recommended concentration: 1-3 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DBH, DBM, Dopamine beta-hydroxylase
• Note: For research use only

DBH antibody (2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.