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DBH/Dopamine Beta Hydroxylase Antibody

SKU: orb1539385

Description

DBH/Dopamine Beta Hydroxylase Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution rangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
ReactivityBovine, Guinea pig, Hamster, Human, Mouse, Rabbit, Rat
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line Jurkat (calculated MW of 69.1kD according to NP_000778.3). Recommended concentration: 1-3 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-DNHYSPHFQEIR, from the internal region of the protein sequence according to NP_000778.3.
TargetDBH/Dopamine Beta Hydroxylase
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
DisclaimerFor research use only

Alternative Names

DBH, DBM, Dopamine beta-hydroxylase

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DBH/Dopamine Beta Hydroxylase Antibody

DBH antibody (2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

DBH/Dopamine Beta Hydroxylase Antibody (orb1539385)

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50 μg
$ 690.00
DispatchUsually dispatched within 2-3 weeks
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