DBH Antibody

• Catalog Number: orb1248744
• Category: Antibodies
• Description: DBH Antibody
• Target: DBH
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DNHYSPHFQEIR
• UniProt ID: P09172
• MW: Approx 70 kDa
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 69.1kDa according to NP_000778.3). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DBH, dopamine beta-hydroxylase (dopamine beta-mono
• Note: For research use only
• NCBI: NP_000778.3

orb1248744 (2 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248744 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.