CYP2C9 Antibody

SKU: orb1257156

Description

CYP2C9 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 211-490 of human CYP2C9 (NP_000762.2).
• Target: CYP2C9
• Molecular Weight: Observed: 63kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CYP2C9, CPC9, CYP2C, CYP2C10, MGC149605, MGC88320, P450 MP-4, P450 PB-1, P450IIC9, CYPIIC9

Western blot analysis of extracts of various cell lines, using CYP2C9 antibody (orb1257156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded mouse lung using CYP2C9 Antibody (orb1257156) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using CYP2C9 antibody (orb1257156). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

CYP2C9

UniProt

P11712