CYP2C9 Rabbit Polyclonal Antibody

SKU: orb5951

Description

CYP2C9 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Drug Metabolism, Lipid Metabolism, Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP2C9 (351-450/490aa)
• Target: CYP2C9
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cytochrome P450 2C9; The Cytochrome P450(P450) superfamily of enzymes is one of three enzyme CPC9; CYP2C; CYP2C10; CYP2C9; Cytochrome p4502C9; P450 MP; P450 PB 1; P450 PB1; P450IIC9; P450MP; S mephenytoin 4 hydroxylase; CP2C9_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP2C9) Polyclonal Antibody, Unconjugated (orb5951) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Stomach tissue lysates, Primary: Anti-CYP2C9 (orb5951) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 52 kDa.

Quick Database Links

Gene Symbol

CYP2C9

UniProt

P11712

Qian Wang1 Junjun Han1 Guiqin Liu1 Yabin Duan2 Delong Duo3 Junbo Zhu1 Yue Lin1 Yawen Xin1 Xiangyang Li1 DNA methylation regulation of CYP450-lipid metabolism by high-altitude hypoxia: linking neuroinflammation to cognitive impairment researchsquare.com, (2025)

PubMed: