CYP2C9 Rabbit Polyclonal Antibody

• Catalog Number: orb5951
• Category: Antibodies
• Description: CYP2C9 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP2C9 (351-450/490aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: CYP2C9
• UniProt ID: P11712
• RRID: AB_10937117
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cytochrome P450 2C9; The Cytochrome P450(P450) sup
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP2C9) Polyclonal Antibody, Unconjugated (orb5951) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Stomach tissue lysates, Primary: Anti-CYP2C9 (orb5951) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 52 kDa.