Chk2 rabbit pAb

• Catalog Number: orb764849
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Chk2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human CHEK2. AA range:35-84
• MW: 61
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CHEK2 antibody, anti-CDS1 antibody, anti-CHK2
• Note: For research use only

Western blot analysis of Chk2 Polyclonal Antibody, using Hela, HCT116, SW480, HEK293 cell, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using Chk2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Western Blot analysis of HuvEc cells using Chk2 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk2 Antibody. The picture on the right is blocked with the synthesized peptide.