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CD74 Antibody: RPE
Catalog Number: orb396368
| Catalog Number | orb396368 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD74 conjugated to RPE . |
| Target | CD74 |
| Clonality | Monoclonal |
| Species/Host | Mouse |
| Isotype | IgG1 |
| Conjugation | RPE |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Purification | Protein G Purified |
| Immunogen | Human CD74 invariant chain synthetic peptide. |
| UniProt ID | P04233 |
| MW | 33 kDa |
| Tested applications | ICC, IF, IHC, WB |
| Dilution range | WB (1:1000); FACS (1:50) |
| Application notes | A 1:1000 dilution of SMC-268 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. |
| Clone Number | 3D7 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Alternative names | DHLAG antibody, HLA DR gamma antibody, HLADG antib Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Entrez | 972 |
| NCBI | NP_001020329.1 |
| Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to heat shock at 42°C for 2 hours and transferred to CO2 incubator at 37°C for 1 hour before trypsinization.
Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
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