CD20 Antibody Cocktail

SKU: orb385680

Description

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). The antibody recognizes the extracellular domain of the protein. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 5-10ul/10^6 cells,Immunofluorescence: 1:50-1:100,Immunohistochemistry (FFPE): 1:100-1:200 for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD20 antibody cocktail to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: L26 + IGEL/733
• Immunogen: Human tonsil B cells (L26) and recombinant protein (IGEL/733) were used as the immunogen for this CD20 antibody cocktail.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Disclaimer: For research use only

IHC testing of CD20 antibody and FFPE human tonsil tissue. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC testing of CD20 antibody and human lymphoma tissue. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of human Raji cells with CD20 antibody cocktail (clones L26 + IGEL/733); Red = isotype control, Blue = CD20 antibody cocktail.

Immunofluorescent staining of human Raji cells with CD20 antibody cocktail (clones L26 + IGEL/733, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P11836