Caspase-4 rabbit pAb

• Catalog Number: orb770578
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Caspase-4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase-4. AA range:75-124
• MW: 45
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CASP4 antibody, anti-ICH2 antibody, anti-Casp
• Note: For research use only

Immunohistochemistry analysis of Caspase-4 antibody in paraffin-embedded human lung carcinoma tissue.

Western blot analysis of lysates from 1) Hela , 2) THP-1, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.