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Item 1 of 4
Item 1 of 4
| Catalog Number | orb763894 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Cleaved-Caspase-4 p20 (Q81) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 4. AA range:62-111 |
| MW | 43+22 |
| Tested applications | ELISA, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-CASP4 antibody, anti-ICH2 antibody, anti-Casp Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of 3T3 cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000.
Western blot analysis of lysates from Jurkat cells, using Caspase 4 (p20, Cleaved-Gln81) Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
