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Product Overview
Product Name BPG 12-step Protein Ladder
Catalog Number orb90562
Tested applications SDS-PAGE, WB
Hazard Information Non-Toxic
Target Protein
Alternative Names
Product Properties
Form/Appearance Approximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Note For research use only.
Product Description

The BPG 12-step Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BPG 12-step Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Application Notes
Application Notes Experiment Notes:

1. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

2. 1.5-2.5 μl per well for general Western transferring.

3. Apply more for thicker (> 1.5 mm) or larger gel.

Validation Images
Migration patterns of BPG 12-step Protein Ladder in different electrophoresis conditions.
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