AURKA Antibody

SKU: orb1263547

Description

AURKA Antibody

Images & Validation

• Tested Applications: FC, IF, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This Aurora-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-392 amino acids from the C-terminal region of human Aurora-A.
• Target: AURKA
• Molecular Weight: 46 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA

Western blot analysis of anti-Aurora-A Pab in A2058 cell line lysate.

Western blot analysis of Aurora-A using rabbit polyclonal Aurora-A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Aurora-A gene (Lane 2).

Confocal immunofluorescent analysis of Aurora-A Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

AURKA

UniProt

O14965

RefSeq:O14965