APTX Antibody

• Catalog Number: orb1257530
• Category: Antibodies
• Description: APTX Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 40kDa
• Target: APTX
• UniProt ID: Q7Z2E3
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: APTX, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using APTX antibody (orb1257530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of A549 cells using APTX antibody (orb1257530).

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug APTX antibody (orb1257530). Western blot was performed from the immunoprecipitate using APTX antibody (orb1257530) at a dilution of 1:500.