APOLIPOPROTEIN J Antibody

• Catalog Number: orb345283
• Category: Antibodies
• Description: CLU antibody
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
• Immunogen: apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
• UniProt ID: P10909
• Tested applications: ELISA, IHC, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:10,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:5,000 - 1:10,000
• Application notes: Anti-apolipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
• Antibody Type: Primary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: goat anti-Apolipoprotein J antibody, Clusterin, Co
• Note: For research use only
• NCBI: NP_001822.3

Western Blot of Anti-Apolipoprotein J Antibody. E-LPs attenuate the increased secretion of α2-macroglobulin induced by NMDA in primary cultures of mixed retinal cells. (C) Conditioned media (HBSS + PBS, n = 5 cell cultures; HBSS + E-LP, n = 5 cell cultures; NMDA + PBS, n = 6 cell cultures; and NMDA + E-LP, n = 6 cell cultures) of the mixed retinal cells were collected 24 hours after treatment with HBSS or 300 µm NMDA treatment with PBS or 300 ng/mL E-LP, and subjected to immunoblotting with antibodies directed against α2-macroglobulin or apo J. The protein levels were normalized by the corresponding apo J levels. Data are means ± SD. *, P = 0.0007 for HBSS + PBS versus NMDA + PBS. #, P < 0.0001 for NMDA + PBS versus NMDA + 300 ng/mL E-LP. Data were analyzed with the 1-way ANOVA, Tukey's multiple comparisons test.

Western Blot of Anti-Apolipoprotein J Antibody. E-LPs reduce the expression and secretion of α2-macroglobulin in primary cultured Müller glia. (C) Conditioned media C of Müller glia were collected 24 hours after 0, 100, or 300 ng/mL E-LP treatment (n = 5 cell cultures), and subjected to immunoblotting with antibodies directed against α2-macroglobulin, apolipoprotein J, apolipoprotein E, or β-actin. The protein levels were normalized by the corresponding apo J C or β-actin D levels. Data are means ± SD. C *, P = 0.0483 or **, P = 0.0059 for 0 ng/mL E-LP versus 100 or 300 ng/mL E-LP, respectively. D *, P < 0.0001 for 0 ng/mL E-LP versus 100 or 300 ng/mL E-LP. Data were analyzed with the 1-way ANOVA, Tukey's multiple comparisons test.