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Catalog Number | orb737325 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to MCHR2 |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
UniProt ID | Q969V1 |
Tested applications | IF, WB |
Dilution range | WB: 1/500 - 1/1000, IF: 1/50 - 1/200 |
Clone Number | MCHR2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Anti-GPR145 antibody, anti-SLT antibody, anti-Mela Read more... |
Note | For research use only |
Western blot analysis of MCH Receptor 1 expression in rat testis (A), BV2 (B), LOVO (C), HCT116 (D) whole cell lysates.
Immunofluorescent analysis of MCH Receptor 1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, IH, WB | |
Human | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |