AK4 Antibody

• Catalog Number: orb1258317
• Category: Antibodies
• Description: AK4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 27kDa
• Target: AK4
• UniProt ID: P27144
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AK3L1, AK3, AK4, AK 4, AK3L1, AK3L2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using AK4 antibody (orb1258317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using AK4 antibody (orb1258317) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using AK4 antibody (orb1258317). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug AK4 antibody (orb1258317). Western blot was performed from the immunoprecipitate using AK4 antibody (orb1258317) at a dilution of 1:1000.