AK4 Antibody

SKU: orb1258317

Description

AK4 Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4 (NP_037542.1).
• Target: AK4
• Molecular Weight: Observed: 27kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

AK3L1, AK3, AK4, AK 4, AK3L1, AK3L2

Western blot analysis of extracts of various cell lines, using AK4 antibody (orb1258317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using AK4 antibody (orb1258317) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using AK4 antibody (orb1258317). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of HepG2 cells, using 3 ug AK4 antibody (orb1258317). Western blot was performed from the immunoprecipitate using AK4 antibody (orb1258317) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

AK4

UniProt

P27144