14-3-3 ζ rabbit pAb Antibody

SKU: orb764418

Description

Rabbit polyclonal antibody to 14-3-3 zeta

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
• Molecular Weight: 28
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-YWHAZ antibody, anti-14-3-3 protein zeta/delta antibody, anti-Protein kinase C inhibitor protein 1 antibody, anti-KCIP-1 antibody

Western Blot analysis of various cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of COLO205 cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of 293T HELA using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.