14-3-3 ζ (phospho Ser58) rabbit pAb

• Catalog Number: orb764378
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 14-3-3 zeta (phospho-Ser58)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73
• MW: 30
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-YWHAZ antibody, anti-14-3-3 protein zeta/delt
• Note: For research use only

Western Blot analysis of various cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 zeta (Phospho-Ser58) Antibody.

Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.