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Catalog Number | orb1256347 |
---|---|
Category | Antibodies |
Description | ZBTB25 Antibody |
Target | ZBTB25 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 266-435 of human ZBTB25 (NP_008908.2). |
UniProt ID | P24278 |
MW | Observed: 49kDa |
Tested applications | IF, WB |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | ZBTB25, KUP, ZNF46, C14orf51 |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using ZBTB25 antibody (orb1256347) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of A549 cells using ZBTB25 antibody (orb1256347). Blue: DAPI for nuclear staining.
WB | |
Bovine, Canine, Equine, Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |