ZAP-70 (phospho Tyr315) rabbit pAb

SKU: orb770406

Description

Rabbit polyclonal antibody to ZAP-70 (phospho-Tyr315)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr315. AA range:281-330
• Molecular Weight: 60
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-ZAP70 antibody, anti-SRK antibody, anti-Tyrosine-protein kinase ZAP-70 antibody, anti-70 kDa zeta-chain associated protein antibody, anti-Syk-related tyrosine kinase antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ZAP-70 (Phospho-Tyr315) Antibody.

Immunohistochemistry analysis of paraffin-embedded human lymph node, using ZAP-70 (Phospho-Tyr315) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with Ca + 40nM 30', using ZAP-70 (Phospho-Tyr315) Antibody. The lane on the right is blocked with the phospho peptide.