YB-1 rabbit pAb

• Catalog Number: orb769293
• Category: Antibodies
• Description: Rabbit polyclonal antibody to YB-1
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human YBX1. AA range:68-117
• Concentration: 1 mg/ml
• Dilution range: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 36
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-YBX1 antibody, anti-NSEP1 antibody, anti-YB1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Western blot analysis of lysates from Jurkat cells, using YB1 Antibody. The lane on the right is blocked with the synthesized peptide.