Wnt-1 rabbit pAb Antibody

SKU: orb766581

Description

Rabbit polyclonal antibody to Wnt-1

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human WNT1. AA range:301-350
• Molecular Weight: 45
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-WNT1 antibody, anti-INT1 antibody, anti-Proto-oncogene Wnt-1 antibody, anti-Proto-oncogene Int-1 homolog antibody

Western Blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 Antibody. The picture on the right is blocked with the synthesized peptide.