Wnt-1 rabbit pAb

• Catalog Number: orb766581
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Wnt-1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human WNT1. AA range:301-350
• MW: 45
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-WNT1 antibody, anti-INT1 antibody, anti-Proto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Western Blot analysis of HT29 cells using Wnt-1 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 Antibody. The picture on the right is blocked with the synthesized peptide.