ULK-101

SKU: orb1301818

Description

ULK-101

Research Area

Cell Biology

Key Properties

• CAS Number: 2443816-45-1
• MW: 460.45
• Purity: 99.66%
• Formula: C₂₂H₁₆F₄N₄OS
• SMILES: Fc1ccc(cc1)-c1cnc2c(cnn2c1)-c1csc(c1)C(=O)N[C@@H](C1CC1)C(F)(F)F
• Target: Autophagy
• Solubility: DMSO:5 mg/mL (10.86 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.17 mM);H2O:Insoluble

Bioactivity

• Target IC50:
  ULK2:30 nM (cell free)|ULK1:8.3 nM (cell free)
• In Vitro:
  U2OS cells were treated with AZD8055 for 2.5 hr, a catalytic mTOR inhibitor that strongly induces autophagy in U2OS cells and observed tha the abundance of DFCP1-positive puncta increased over 3-fold. Co-treatment of cells with 5 μM ULK-101 completely abrogate the formation of DFCP1-positive structures induced by AZD8055 treatment, consistent wit the loss of Beclin 1 phosphorylation.
• Cell Research:
  Cells (U2OS or NSCLC) were seeded on tissue culture treated 96-well plates at 1,000 cells per well in RPMI-1640 media supplemented with 10% FBS. Twenty-four hours later, media was aspirated, wells rinsed with 1x DPBS, and replaced with full media (FM) or Optistarve (OS) with a concentration gradient of ULK-101 final concentration of 100 μM, 50 μM, 25 μM, 12.5 μM, 6.25 μM, 3.1 μM, 1.6 μM, 0.8 μM, 0.4 μM, or 0 μM). Two days later, media and ULK-101) was aspirated, wells were rinsed with 1x DPBS, and All wells replaced with FM. Five days later, relative ATP levels were measured using a luminescent CellTiter-Glo assay.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, T8853, ULK, ULK 101, ULK1, ULK-101, ULK101, Unc-51 like kinase

Compound Identifiers

PubChem CID

137628686