UBE3C Antibody

SKU: orb1257007

Description

UBE3C Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human UBE3C (NP_055486.2).
• Target: UBE3C
• Molecular Weight: Observed: 124kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

HECTH2, ubiquitin-protein ligase E3C, HECT-type ubiquitin transferase E3C, ubiquitin-protein isopeptide ligase (E3)

Western blot analysis of extracts of various cell lines, using UBE3C antibody (orb1257007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast cancer using UBE3C Antibody (orb1257007) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using UBE3C antibody (orb1257007). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

UBE3C

UniProt

Q15386