UBE3C Antibody

• Catalog Number: orb1257007
• Category: Antibodies
• Description: UBE3C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human UBE3C (NP_055486.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 124kDa
• Target: UBE3C
• UniProt ID: Q15386
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HECTH2, ubiquitin-protein ligase E3C, HECT-type ub
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using UBE3C antibody (orb1257007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast cancer using UBE3C Antibody (orb1257007) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using UBE3C antibody (orb1257007). Blue: DAPI for nuclear staining.